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Protocol |
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Before use, centrifuge the B-PE suspension at 10,000g for 10 min at 4°C. Discard the supernatant. Resuspend the pellet (B-PE) into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis. Store B-PE at 4°C and keep away from light.
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Substrate/Buffer |
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B-PE is supplied in sodium phosphate buffer, pH 7.0 with ammonium sulfate.
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References |
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1. Glazer, AN. and CS. Hixson, J. Biol. Chem. 252, 32 (1977).
2. Lundell, DJ. et al. J. Biol. Chem. 259, 5472 (1984).
3. Oi, VT. et al. J. Cell Biol. 93, 981 (1982).
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Notes |
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B-PE (B-Phycoerythrin), a fluorescent protein, belongs to the phycobiliprotein family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. B-PE is made of α, β and γ subunits and is present as (αβ)6γ. The absorption bands peak at 545 nm (εM =2.41 X 106 M-1cm-1) and 563 (εM =2.33 X 106 M-1cm-1).1,2 B-PE and the closely related R-PE are the most intensely fluorescent phycobiliproteins. They are significantly brighter and more photostable than conventional organic fluorophores.3 B-PE labeled streptavidins, primary and secondary antibodies have been widely used in applications such as flow cytometry and multi-color immunofluorescent staining.
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