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SampleTypes |
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cytospin, cell smear, cells, tissue sections
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Specificity |
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Precise, fast, and simple technique for detecting and quantitating apoptotic DNA fragmentation at a single-cell level in cells and tissues with a red fluorescent label for fluorescence microscopy and flow cytometry
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Notes |
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The In Situ Cell Death Detection Kit, TMR red is based on the detection of single- and double-stranded DNA breaks that occur at the early stages of apoptosis.
Apoptotic cells are fixed and permeabilized. Subsequently, the cells are incubated with the TUNEL reaction mixture that contains TdT and TMR-dUTP. During this incubation period, TdT catalyzes the addition of TMR-dUTP at free 3'-OH groups in single- and double-stranded DNA. After washing, the label incorporated at the damaged sites of the DNA is visualized by flow cytometry and/or fluorescence microscopy.
Only Sweden
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