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Alias names |
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Dicarbonyl/L-xylulose reductase
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Concentration |
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0.5 mg/ml (determined by Bradford assay)
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Substrate/Buffer |
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Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50mM NaCl
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Storage |
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Can be stored at +4°C short term (1-2 week). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles
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References |
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Cho Veqa JH., et al. (2007) Cancer Epidemiol Biomakers Prev. 16(12):2615-22. Nakaqawa J., et al. (2002) J Biol Chem. 277(20):17883-91.
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Notes |
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Dicarbonyl/L-xylulose reductase, also known as DCXR, is an enzyme responsible for the metabolism of xylulose, converting it into xylitol. DCXR was expressed at low levels and was localized predominantly in the cytoplasmic membrane. In contrast, in virtually all grades of early-stage prostate cancer and in all chemohormonally treated cases, DCXR was strikingly overexpressed and was localized predominantly in the cytoplasm and nucleus. Recombinant human DCXR, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
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