|
Alias names |
|
Glutaredoxin-related protein 5 mitochondrial, C14orf87, GRX5
|
|
Sequence |
|
MGSSHHHHHH SSGLVPRGSH MSGSLGRAAA ALLRWGRGAG GGGLWGPGVR AAGSGAGGGG SAEQLDALVK KDKVVVFLKG TPEQPQCGFS NAVVQILRLH GVRDYAAYNV LDDPELRQGI KDYSNWPTIP QVYLNGEFVG GCDILLQMHQ NGDLVEELKK LGIHSALLDE KKDQDSK
|
|
Concentration |
|
0,5 mg/ml (determined by Bradford assay)
|
|
Substrate/Buffer |
|
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl
|
|
Storage |
|
Can be stored at +4°C short term (1-2 week). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles
|
|
References |
|
Holmgren A., et al. (1988) FEMS Microbiol. Rev. 4 (4): 271–297.
Holmgren A., et al. (1988) Biochem. Soc. Trans. 16 (2): 95–96
|
|
Notes |
|
GLRX5 is small redox enzymes of approximately one hundred amino-acid residues which uses glutathione as a cofactor. This protein is oxidized by substrates, and reduced non-enzymatically by glutathione. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Recombinant ㅗhuman GLRX5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
|