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DAPI - Detailed product view

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Order Nr D3449
Description 4’,6-Diamidino-2-phenylindole, Dihydrochloride
Sizes 10mg
Format yellow powder
Source/Host Synthetic
Applications FC
MW 350.30
Alias names   DAPI, 2-(4-Amidinophenyl)-6-indolecarbamidine, Dihydrochloride
Purity   ~90%
Storage   May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Contents/Specifications   Melting Point: >300°C

Atmosphere: Inert Gas

Solubility: Water, Methanol, DMSO

Stability: Light Sensitive

Method for Determining Identity:
Proton NMR Spectroscopic Analysis

TLC Conditions:
SiO2: Dichloromethane: Methanol; Acetic Acid; 2: 2: 1; Visualized with UV and AMCS; Elongated spot; Rf=0.5-0.75.
Chloride content: 21.8%. Contains 3% methanol by NMR.

Molecular Formula: C16H17Cl2N5
Notes   A cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
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