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Specificity |
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For use in fluorescence in situ hybridization (FISH) procedures.
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Protocol |
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After hybridization and wash steps of a FISH experiment, pipette DAPI/Antifade Solution EjqNF onto the slide (for example 30 μl onto an area of 24 mm x 60 mm). Avoiding trapped bubbles, cover the samples with a coverslip and incubate in the dark for 15 min. Using a pipette tip which has been cut off to increase the size of the opening, can make the pipetting process easier. Avoid long exposure to light. Carefully remove excess DAPI/Antifade-Solution EjqNF by gently pressing the slide between filter papers.
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Storage |
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The component must be stored at -16-22°C, protected from light.
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Contents/Specifications |
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MT1, DAPI/Antifade-Solution, 0.8 ml, Reaction vessel, blue lid.
Component EjqNF is sufficient for approx. 20 reactions.
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