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Specificity |
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analysis of Frataxin quantity
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Protocol |
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The Frataxin FlexPlex™ Module is used to determine the quantity of Frataxin protein in a sample. Frataxin is immunocaptured within the wells. The quantity of protein is measured by adding a second Frataxin specific antibody (1X Detector Antibody) which is then labeled with horseradish peroxidase (1X HRP Label). This peroxidase changes the substrate from colorless to blue. The rate of color development is proportional to the amount of protein captured in the well and can be monitored at 600 nm. Alternatively, the assay can be terminated, at a user defined time, by the addition of 1N HCl (not supplied) and the assay performed as an end point measurement at 450 nm
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Storage |
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Store all tubes and the microplate strips at 4°C. The kit is stable for 6 months
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Contents/Specifications |
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8209822 10X Detector Antibody 0.2 mL
8209823 10X HRP Label 0.2 mL
8209824 Microplate strips (4-pack) 1
The FlexPlex™ Starter Kit (MSFX-ST) is required for this assay
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