|
Specificity |
|
Mouse Anti-Human CD123 monoclonal antibody was raised by immunizing mouse with Human IL-3Rα transfected COS cells and screened for anti-human CD123.
|
|
Protocol |
|
Applications include flow cytometry, Western blotting1, immunoprecipitation1, and immunohistochemical staining2 of acetone-fixed frozen sections. Recommended usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 μg per million cells in 100 μl volume or 100 μl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
|
|
Substrate/Buffer |
|
The vial contains 0.25 ml antibody (at 200μg/ml) in 1X PBS (pH 7.2) with 0.09% sodium azide.
|
|
Storage |
|
Stored at 4°C. for up to 1 year. Avoid repeated freezing and thawing.
|
|
References |
|
1. Sun, Q. et al., 1996. Blood 87:83.
2. Herling, M., et al., 2003. Blood 101:5007.
|
|
Notes |
|
CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
|
|
Related products |
|
Streptavidin; HiLyte Fluor 680 labeled, Cat # 108-60659-H680
Streptavidin; HiLyte Fluor 750 labeled, Cat # 108-60659-H750
Streptavidin; HiLyte Fluor 488 labeled, Cat # 108-60665
Streptavidin; HiLyte Fluor 647 labeled, Cat # 108-60667
Streptavidin; HiLyte Fluor 555 labeled, Cat # 108-60666
Streptavidin; HiLyte Fluor TR labeled, Cat # 108-60671
Streptavidin; HiLyte Plus 555 labeled, Cat # 108-60672-Plus555
Streptavidin; HiLyte Plus 647 labeled, Cat # 108-60672-Plus647
Streptavidin; HiLyte Plus 750 labeled, Cat # 108-60672-Plus750
|