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Protocol |
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WB 1:1,000 - 10,000
IHC 1:250 - 500
IP 1:10 - 100
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Substrate/Buffer |
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50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA
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References |
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1. Bajorath J, et al. Protein Sci 4(8): 1644-7, 1995
2. Robinson WH, et al. Eur J Immunol 25(10):2765-9, 1995
3. Ibanez A, et al. J Immunol 177(2): 1152-9, 2006
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Notes |
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CD6 is a monomeric T-cell surface glycoprotein that is involved in T-cell activation (1). It is predominantly expressed on thymocytes, subpopulations of B-cells, and mature T-cells. Through its binding to activated leukocyte cell adhesion molecule (ALCAM/CD166), CD6 is considered to play an important role in lymphocyte development and activation. CD6 is a constitutively phosphorylated molecule that becomes hyperphosphorylated by serum and protein kinase C (PKC) activators, causing a molecular mass shift from 105 to 130 kDa (2,3).
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