Transcriptor One-Step RT-PCR Kit
High Sensitivity for Detecting RNA
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Obtain high sensitivity and yield in a wide amplification range:
Detect as little as 1 fg of total RNA or 1 copy of viral RNA (in vitro transcripts) (Figures 1 and 2).
Achieve high specificity and reduce primerdimers:
Count on excellent performance with the kit’s proprietary hot start buffer (patents pending, Figure 2).
Produce long fragments:
Generate transcripts up to 6.5 kb (Figure 3).
Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA):
Overcome high secondary structure by reverse transcribing at 50°C with thermostable Transcriptor Reverse Transcriptase in combination with an optimized buffer (Figure 1).
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Figure 1: Transcribe difficult templates with high sensitivity.
Various amounts (down to 1 fg) of total HeLa RNA were reverse transcribed with the Transcriptor One-Step RT-PCR Kit. A 389 bp fragment (GC content is 64%) was amplified with specific primers for human 28S ribosomal RNA according to the kit’s standard RT-PCR protocol (reverse transcription at 50°C for 30 minutes). As shown by the clearly visible band obtained after agarose gel electrophoresis and ethidium bromide staining, the kit transcribes even small amounts (1 fg) of template with high sensitivity.
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Save time and improve results with the new Transcriptor One-Step RT-PCR Kit.
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Minimize pipetting steps and prevent contamination
by using a premixed enzyme blend and optimized buffer that includes PCR-grade dNTPs and hot start components.
Protect your RNA from degradation by RNases
with the supplied thermostable Protector RNase Inhibitor that protects the RNA at high temperatures (up to 60°C), where RNases could be a problem.
Use a convenient one-step format for efficient,
reproducible RT-PCR using total RNA, mRNA, in vitrotranscribed RNA, or viral RNA as starting material.
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Figure 2: Achieve high sensitivity and specificity with the Transcriptor One-Step RT-PCR Kit.
A 389 bp fragment of human 28S ribosomal RNA (A) or a 2.5 kb fragment of human ApoB (B) was amplified with Transcriptor One-Step RT-PCR Kit or one-step RT-PCR kits from other suppliers using manufacturers’ recommended reaction conditions. Results show that the Transcriptor One-Step RT-PCR Kit efficiently amplifies a wide range of sample amounts with greater sensitivity and specificity compared to the other suppliers’ kits.
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Figure 3: Generate fragments up to 6.5 kb.
Various amount of total or viral RNA were reverse transcribed with the Transcriptor One-Step RT-PCR Kit using the reaction conditions outlined in the package insert. Samples: 106 copies of hepatitis A virus (HAV) RNA (246 bp fragment); 10 ng of total RNA of different tissue types or K-562 cells (139 bp, 387 bp, 587 bp, and 1.8 kb fragments); 100 ng of total RNA from different tissue types or HeLa cells (2.5 kb, 3.6 kb, 5.3 kb, 6.0 kb, and 6.3 kb fragments). Results show that the Transcriptor One-Step RT-PCR Kit efficiently transcribes a broad range of fragment sizes.
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Product
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Catalog No.
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Pack Size
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Transcriptor One-Step RT-PCR Kit
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04655877001 |
1 kit (50 reactions including 10 control reactions) |
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04655885001
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1 kit (150 reactions) |
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Add convenience and sensitivity to your one-step RT-PCR experiments by requesting a free sample of the Transcriptor One-Step RT-PCR Kit today.
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