AnaTag™ APC, B-PE and R-PE Labeling Kits – New & Improved
Labeling of antibodies can be both tedious and time-consuming. Without the use of proper techniques and reagents, the conjugation may result in suboptimal or no labeling. AnaSpec’s improved APC, B-PE and R-PE Labeling Kits contain all the reagents needed for optimized labeling of antibodies. The phycobiliproteins, modified by SMCC, result in the creation of SH-reactive moieties, which can easily react with the thiol groups of target antibody. Since no additional activation is required, the conjugation process is simple and easy.
Phycobiliproteins are a family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. Allophycocyanin (APC) is an ultra-sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm. R-Phycoerythrin (R-PE), made of a, b and g subunits and present as (ab)6g, has broad absorption bands with peaks at 565 nm (eM =1.96 X 106 M-1cm-1), 498 (eM =1.53 X 106 M-1cm-1), and 539 nm (eM =1.62 X 106 M-1cm-1. These phycobiliproteins are significantly more sensitive and photostable than conventional organic fluorophores. APC, B-PE or R-PE labeled antibodies are used in applications such as flow cytometry and immunofluorescence.
AnaTag™ kits provide all the reagents, purification column needed to label up to 1 mg of antibody. A detailed step-by-step protocol is also included. APC, B-PE or R-PE and SMCC activated derivatives of these proteins can also be purchased separately.
|
|
|
|
Figure 1. Tubulin in bovine pulmonary artery endothelial cells were detected with mouse anti-alpha-tubulin primary antibody, visualized with APC-conjugated goat anti mouse IgG. Nuclei were stained with DAPI (cat# 83210)
|
Figure 2. Actins in bovine pulmonary artery endothelial cells were detected with biotin-conjugated phalloidin, visualized with R-PE-conjugated streptavidin (cat#60669). Nuclei were stained with Hoechst 33342 (cat# 83218). |
Protein Labeling Kits
|
Product
|
Ex/Em (nm/nm)
|
Size
|
Cat #
|
|
AnaTag™ APC Labeling Kit NEW
|
650/660
|
1 kit
|
72111
|
|
AnaTag™ B-PE Labeling Kit NEW
|
545, 563/578
|
1 kit
|
72112
|
|
AnaTag™ R-PE Labeling Kit NEW
|
565, 498, 539/578
|
1 kit
|
72113
|
APC, B-PE and R-PE Products
|
Product
|
Size
|
Cat #
|
|
B - PE (B - Phycoerythrin)
|
1 mg
|
82001
|
|
CL - APC (Cross Linked - Allophycocyanin)
|
1 mg
|
82002
|
|
R - PE (R - Phycoerythrin)
|
1 mg
|
82004
|
|
SMCC Activated B - PE (B - Phycoerythrin) NEW
|
1 mg
|
72109
|
|
SMCC Activated CL - APC (Cross Linked - Allophycocyanin) NEW
|
1 mg
|
72108
|
|
SMCC Activated R - PE (R - Phycoerythrin) NEW
|
1 mg
|
72110
|