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Basophils and mast cells

2009-03-31

Unique antibodies fro functional studies on mouse basophils and mast cells


 

Ba103 and Ba91, antibodies recognizing CD200R3: important new tools to study the mysterious roles of basophils (Cat.# HM1103 and HM1104)

Special features

  • mAb Ba103 is useful for depletion of basophils but not mast cells

  • mAb Ba91 and mAb Ba103 added to basophils or mast cells induce degranulation of the cells in vitro

  • bulk quantity available of 1 mg (Cat.# HM1103b; HM1104b)

    • More info

  • CD200R3 functions as an activating receptor on mast cells and basophils

  • CD200R3 regulates IgE-independent immune responses in cooperation with an inhibitory receptor CD200R
     
  • Basophils are candidate effector cells for the immune response against parasites

  • Basophils are a major source of the typical Th2 cytokine IL-4 during parasite infections
    • Figure 1. mAb Ba91 induced degranulation of MC/9 cells. MC/9 cells were incubated with the indicated reagents. Degranulation was calculated by measuring β-hex osaminidase release.
     
    Cat.# Product name Quantity Applications
    HM1103 Monoclonal antibody against CD200R3, clone Ba103 100 µg WB, IP, FC, FS
    HM1104 Monoclonal antibody against CD200R3, clone Ba91 100 µg WB, IP, FC, FS
    HM1103b Monoclonal antibody against CD200R3, clone Ba103 1 mg WB, IP, FC, FS
    HM1104b Monoclonal antibody against CD200R3, clone Ba91 1 mg WB, IP, FC, FS
     

    References

    1. Kojima, T et al; Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner. J Immunol 2007, 179: 7093

       

    2. Obata, K et al; Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood 2007, 110: 913

       

    3. Karasuyama, H et al; Newly discovered roles for basophils: a neglected minority gains new respect. Nature Rev Immunol 2009, 9: 9

       

    4. Sullivan, B et al; Basophils: a nonredundant contributor to host immunity. Immunity 2009, 30: 12

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